The router has a built-in firewall that will control access to each PC individually. Even if you forward port 5060 for one PC, on another PC it will still be blocked.
Also, you should make sure that the RTP port setting (under Options -> Network tab) should be a high value, for example 8000 - 16000. The low port numbers are more often than not blocked by the router's firewall and this can cause problems receiving/delivering audio.
Also try this, put the call on hold for a few seconds and then take it off hold. Is the audio problem corrected?
Also make sure your stun servers are active, or if you prefer you can manually enter the IP address, SIP and RTP port details.